A genipin crosslinked silk fibroin monolith by compression molding with recovering mechanical properties in physiological conditions

Fibroin monoliths, produced by compression molding starting from the protein powder, are known to achieve an impressive mechanical strength when tested in dry conditions. However, presence of water, loss structural integrity and performances is striking. Considering bone scaffold applications for which this material intended, remarkable weakening after implantation strongly limits its usage non-structural purposes. Here, we propose approach that allows recovery properties retention shape physiological conditions, due a progressive chemical crosslinking triggered genipin humidity. Crosslinking does not hinder cell adhesion proliferation, as suggested preliminary biological test, nor it limit machinability material, demonstrated producing complex-shaped objects through laser cutting. Overall, genipin-crosslinked solid fibroin reliable may be suitable